Methods of using dyes and associated technology are provided. A dye, such
as a monomeric dye or a dimeric dye, may be used in a nucleic acid gel
staining application and/or a nucleic acid detection application. Such a
dye and a salt that comprises an anion that is associated with a strong
acid and a cation that is associated with a strong base may be used in
such an application. A dimeric dye, such as a dimeric dye capable of
forming a hairpin-like structure, may be used to stain and/or detect
nucleic acids via a release-on-demand mechanism. A dimeric dye having low
background fluorescence in the absence of nucleic acids and high
fluorescence in the presence of nucleic acids, upon binding therewith,
may be used to stain and/or detect nucleic acids.