The present invention is directed to methods and compositions for generating a pool of oligonucleotides. The invention finds use in preparing a population or subpopulations of oligonucleotides in solution. The pool of oligonucleotides finds use in a variety of nucleic acid detection and/or amplification assays.

 
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> Fingerprint analysis for a plurality of oligonucleotides

> Cold shock inducible expression and production of heterologous polypeptides
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