A load/unload control system for a tray, the load/unload control system includes a control module and a drive module. The control module is used for controlling a voltage, the control module includes a storage unit for storing instructions and an output unit constructed and arranged for reading the instructions from the storage unit and outputting the instructions. The drive module is coupled to the drive module for supplying the voltage adapted to move the tray based on the instructions, the instructions comprising at least one step-up voltage instruction for increasing the voltage during a load process and at least one step-down voltage instruction for decreasing the voltage during an unload process. A related control method for controlling a voltage to load/unload a tray is also provided.