This invention concerns induction of gene expression specifically in given
tissue with the use of a novel promoter that has environmental stress
responsiveness and functions specifically in given tissue. It is realized
by a method comprising: a step of preparing a plant having an arbitrary
gene downstream of the environmental stress responsive promoter
comprising DNA (a), (b), or (c) below; (a) DNA comprising the nucleotide
sequence as shown in any of SEQ ID NO: 1 to 8; (b) DNA comprising a
nucleotide sequence derived from the nucleotide sequence as shown in any
of SEQ ID NO: 1 to 8 by deletion, substitution, or addition of one or a
plurality of nucleotides and functioning as an environmental stress
responsive promoter; or (c) DNA hybridizing under stringent conditions to
DNA comprising the nucleotide sequence as shown in any of SEQ ID NO: 1 to
8 and functioning as an environmental stress responsive promoter; and a
step of cultivating the plant in the presence of environmental stress,
the method comprising inducing expression of the gene located downstream
of the environmental stress responsive promoter in a tissue-specific
manner.