Disclosed herein is a method for extending the sugar chain modification of
a lipid or a protein by introducing two or more glycosyltransferase genes
in a biological organism (including animal-, plant- or microbial cells).
Also disclosed is a recombinant plant with a potency to generate ceramide
trihexoside and a method for generating ceramide trihexoside using the
plant. Methods for synthetically preparing ceramide trihexoside utilizing
a transgenic tobacco prepared by constructing an expression vector with
the ORF of human- or animal-derived .alpha.1,4-galactosyltransferase gene
(.alpha.1,4 GT) for plants and subsequently introducing the vector into
tobacco using a genetic approach for plants and methods for the
mass-scale production of ceramide trihexoside are also disclosed herein.