A shoelace fastening assembly includes a fastening device fixed on the tongue of a shoe and includes a main part which has a tubular member and a shoelace is wrapped on the tubular member. A knob is rotatably engaged with the main part and drives the tubular member to rotate in one direction. At least one intermediate member is fixed on the tongue and has a cruciform slot so that the shoelace is movably engaged with the cruciform slot. At least two hook members are respectively fixed on two flaps of the shoe and each hook member has an engaging groove with which the shoelace is movably engaged. The shoelace can be easily connected with the intermediate member and the hook members.