An LDO with over-current protection includes a first and a second P-type transistor, a sensing resistor, a comparator, and an error amplifier. The channel aspect ratio of the first P-type transistor is much higher than that of the second P-type transistor. The first P-type transistor generates output voltage source according to input voltage source and current control signal. The sensing resistor is coupled among the input voltage source, the second P-type transistor, and the comparator, providing a sensing voltage. The comparator generates a current limiting signal according to first reference voltage and the sensing voltage. When the current limiting signal enables the error amplifier, the error amplifier adjusts voltage of the current control signal according to second reference voltage and voltage divided from the output voltage source; when the current limiting signal disables the error amplifier, voltage of the current control signal is not adjusted.