A process for the production and maintenance of pluripotent and/or
totipotent progenitor cereal cells from undifferentiated callus cells is
described. Production of the progenitor cells takes place via direct
organogenesis on a medium containing at least one auxin and at least one
cytokinin. For example, the auxin may be 2,4-dichlorophenoxyacetic acid,
indole-3-acetic acid, picloran, naphthelenacetic acid, indole-3-propionic
acid, indole-3-butyric acid, phenyl acetic acid, benzofuran-3-acetic acid
or phenyl butyric acid, and the cytokinin may be benzyl amino purine,
benzyladenine, thidiazuron, zeatin, isopentyladenine, trans-zeatin or
dimethylallyladenine. Processes for transformation of the
undifferentiated callus cells and/or the progenitor cereal cells are also
described. Typical cereal cells are sorghum, maize, wheat, barley,
millet, rye, canola, alfalfa, triticale and rice.