A fungal wild-type lipolytic enzyme having a higher ratio of activity on
polar lipids compared with triglycerides, wherein the enzyme preferably
has a phospholipid:triglyceride activity ratio of at least 4. Preferably,
the lipolytic enzyme according to the present invention has a
glycolipid:triglyceride hydrolyzing activity ratio of at least 1.5. In
one embodiment, the fungal lipolytic enzyme according to the present
invention comprises an amino acid sequence as shown in SEQ ID NO: 1 or
SEQ ID No. 2 or SEQ ID No. 4 or SEQ ID No. 6 or an amino acid sequence
which has at least 90% identity thereto. The present invention further
encompasses a nucleic acid encoding a fungal lipolytic enzyme, which
nucleic acid is selected from the group consisting of: (a) a nucleic acid
comprising a nucleotide shown in SEQ ID No. 3, SEQ ID No. 5 or SEQ ID No.
7; (b) a nucleic acid which is related to the nucleotide sequence of SEQ
ID No. 3, SEQ ID No. 5 or SEQ ID No. 7 by the degeneration of the genetic
code; and (c) nucleic acid comprising a nucleotide sequence which has at
least 90% identity with the nucleotide sequence shown in SEQ ID No. 3,
SEQ ID No. 5 or SEQ ID No. 7.