A localization system and method of a mobile robot using a camera and artificial landmarks in a home and a general office environment (or working zone) is provided. The localization system includes artificial landmarks having an LED flash function in an invisible wavelength band, a camera with a wide-angle lens, a module flashing landmarks attached at the ceiling and identifying positions and IDs of the landmarks from an image photographed by the camera having a filter, a module calculating position and orientation of the robot using two landmarks of the image in a stop state, a module, when a ceiling to which the landmarks are attached has different heights, a position of the robot, and a module, when a new landmark is attached in the working zone, calculating a position of the new landmark on an absolute coordinate.