A biometric identification apparatus and method using bio signals and an artificial neural network, are provided. The biometric identification apparatus includes: a periodic signal extraction unit which extracts one or more periodic signals from an input bio signal; a template calculation unit which calculates a template value using the extracted periodic signals; a template storage unit which stores a plurality of template values corresponding to a plurality of living bodies; and a reading unit which reads the template value that is most approximate to the template value calculated by the template calculation unit from the template storage unit. Accordingly, it is possible to identify a living body by taking into consideration all of the characteristics of bio signals detected from the living body.