According to the invention, there is provided a novel soybean variety designated RJS32001. This invention thus relates to the seeds of soybean variety RJS32001, to the plants of soybean RJS32001 to plant parts of soybean variety RJS32001 and to methods for producing a soybean plant produced by crossing plants of the soybean variety RJS32001 with another soybean plant, using RJS32001 as either the male or the female parent.