An endoscope pre-cleaning kit has a sealed, flexible pouch having front and back sidewall panels and a bottom gusset panel. The sidewall panels are superimposed over one another and sealed together proximate to their top and side peripheral edges to define a top seal and two opposing side seals. The sidewall panels are sealed to the bottom gusset panel proximate to their bottom peripheral edges to define a gusseted bottom portion such that the pouch is adapted to stand upright. The kit also includes a pre-diluted detergent contained within the pouch, and an absorbent pad adapted to absorb a portion of the detergent. The pouch includes a portion adapted to be opened in order to access the detergent.