According to the invention, there is provided a novel soybean variety designated RJS48001. This invention thus relates to the seeds of soybean variety RJS48001, to the plants of soybean RJS48001 to plant parts of soybean variety RJS48001 and to methods for producing a soybean plant produced by crossing plants of the soybean variety RJS48001 with another soybean plant, using RJS48001 as either the male or the female parent.