Variants of Bacillus sp. TS-23 strain alpha-amylases exhibit improved
enzymatic performance, including increased themostability, reduced
calcium dependence, increased washing/cleaning performance, and baking
ability. Compositions comprising these variants are useful in methods of
starch processing, starch liquefaction, fermatation, starch
saccharification, cleaning, laundrying, textile desizing, baking, and
biofilm removal.