The present invention provides: a protein having an improved nitrile
hydratase activity, whereby heat resistance has been improved when
compared with a wild-type nitrile hydratase activity, wherein the amino
acid sequence of a nitrile hydratase is modified; a gene DNA encoding the
above protein; a recombinant vector having the above gene DNA; a
transformant or transductant having the above recombinant vector; a
nitrile hydratase collected from a culture of the above transformant or
transductant, and a production method thereof; and a method for producing
an amide compound.