The present invention is directed to methods and compositions which employ an Arabidopsis VIP1 gene or VIP1-like gene from another plant species for purposes of increasing host plant susceptibility to Agrobacterium infection. Methods for increasing susceptibility of a host to Agrobacterium infection and methods for improving Agrobacterium-mediated gene transfer are provided. Also provided are chimeric genes and expression vectors that, upon introduction into a plant cell, enable the plant cell to become more susceptible to Agrobacterium infection and thus better transformed via Agrobacterium-mediated gene transfer. Transgenic plant cells and plants regenerated therefrom which overexpress a VIP1 or VIP1-like gene are also provided.