The present invention provides a genetic engineering material for insects
that enables a target protein to be purified easily, without requiring
the use of recombinant baculovirus, while simultaneously providing a
process for producing exogenous protein using that genetic engineering
material. A gene recombinant silkworm is obtained by inserting an
exogenous protein gene such as a cytokine gene coupled to a promoter that
functions in silk glands into a silkworm chromosome. An exogenous protein
such as a cytokine is then extracted and purified from the silk glands or
cocoon of that silkworm or its offspring. A large amount of exogenous
protein can be produced within silk gland cells, outside silk gland cells
or in silk thread or a cocoon by inserting an expression gene cassette,
in which the DNA sequence of the 3' terminal portion and the DNA sequence
of the 5' terminal portion of fibroin H chain gene are fused to the
exogenous protein gene, into silk gland cells and so forth.