Disclosed is a method for constructing a nucleus-implanted egg, a
parthenogenetic embryo and for producing a parthenogenetic mammal each
having 2 haploid genome sets originating in mammalian ova, and provides
methods of constructing a nucleus-implanted egg having a haploid genome
set derived from primitive ovarian follicle egg (ng ovum) and a haploid
genome set from MII phase (second meiosis metaphase) egg (fg ovum), a
parthenogenetic embryo and a parthenogenetic mammal, including steps (1)
introducing ng ovum into a nucleus-deleted deleted germinal vesicle stage
(GV) egg, developing the obtained egg to MII phase by in vitro maturing
and culturing to prepare a first nucleus-implanted egg, and (2)
extracting MII phase chromosome from the first nucleus-implanted egg and
introducing it into other fg ovum to prepare a second nucleus-implanted
egg, wherein a ng or fg ovum from which an imprinted gene undergoing
epigenetic modification during sperm generation is used.