The present invention provides methods, kits and compositions for the
detection of an analyte. In the methods of the invention, a complex is
formed between two or more analyte specific probes (ASP) and an analyte.
The analyte specific probes each have a portion of a polymerase which
interact to form a functional polymerase complex upon binding of the ASP
to the analyte. The functional polymerase complex then generates a
detectable signal which is indicative of the presence and/or amount of
the analyte in the sample.