The present invention relates to modification of amplification buffer used in amplification reactions. The modifications result in a significant improvement in results of amplification. In particular, the present invention provides methods and buffers for performing an amplification reaction utilizing a buffer comprising nucleotide triphosphates comprising treating the buffer to substitute a portion of the nucleotide triphosphates with nucleotide diphosphates.

 
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> Arrays of PDZ domain polypeptides

> Activation and expansion of T-cells using an agent that provides a primary activation signal and another agent that provides a co-stimulatory signal
~ 00584