The integration of genes into methylobacterium, such as M. extorquens ATCC
is disclosed, using a transposon system, preferably the mini-Tn7
transposon system, and under the control of a promoter, such as the
strong methanol dehydrogenase promoter (P.sub.mxaF). Multicopy
integration of genes of interest is also disclosed. The unique and
specific attachment site for the Tn7 attachment (attTn7) is identified
for M. extorquens.