A regenerated tobacco material is manufactured through extracting a natural tobacco material with an extracting solvent to obtain an extracted solution containing components of the natural tobacco material and an extraction residue. A regenerated tobacco web is prepared by using the extraction residue. On the other hand, the extracted solution is subjected to a fractionating treatment by means of ultrafiltration, reverse osmosis filtration, or reversed-phase partition chromatography to obtain a first fraction enriched in desired components and depleted in undesired components, and a second fraction enriched in the undesired components and depleted in the desired components. The first fraction is added, optionally together with the second fraction decreased in amount, to the regenerated tobacco web to prepare a regenerated tobacco material.