Disclosed is a system and method for generating quantitative imagery of demyelination in the spinal cord. The method includes acquiring a magnetization transfer weighted (MTw) MR image of the spinal column, identifying a reference region of interest within the image corresponding to cerebrospinal fluid (CSF), averaging the signal intensity corresponding to the reference region of interest, and computing a ratio, on a voxel-by-voxel basis, of the signal intensity of each voxel by the averaged reference signal intensity. In doing so, normalized MTw images are obtained such that detrimental artifacts such as motion-induced errors, coil loading, and RF coil sensitivity variations are obviated.