The present invention provides a virus-like particle (VLP) comprising i) a
polypeptide comprising a polypeptide of interest (POI) and at least a
particle-associating portion of a large envelope (L) polypeptide of an
avian hepadnavirus or a functional derivative or homolog thereof, and ii)
a small envelope (S) polypeptide of an avian hepadnavirus or a functional
derivative or homolog thereof. By introducing one or more POIs into the L
polypeptide, the POI is translocated along with L into a particle
structure made up primarily of S polypeptide. The present invention
furthermore provides methods for producing a recombinant virus-like
particle.