The present invention provides a method for microelectronic positioning bioparticles, which utilizes dielectric and non-contact electrical force of cell themselves associated with multi-phase electrical signals to attain uniformity of distribution of the bioparticles and positioning thereof within micro-areas in a cell culture system. The obstacle of system geometrical structure is eliminated so as to simplify the system layout and programmably change cells' positions. The present method is suitable for treating cell array in a large quantity. The present method utilizes electrical control for the cells. The clamping of the cells can be removed at any time. It is very advantageous for collection and redistribution of cell products.