A pretreatment kit and a pretreatment kit for saliva in identification and
quantitative determination of Streptococci mutans by immunochromatography
utilizing an antigen-antibody reaction, which can remove aggregation
caused by mucin and chain formation of Streptococci mutans in saliva in a
simple operation and can efficiently flow out a complex of a labeled
antibody and Streptococci mutans from a porous membrane retaining the
labeled antibody, contains (A) a 0.01 to 10 mol/L aqueous solution of
sodium hydroxide, (B) a 0.01 to 3 mol/L aqueous solution of tartaric acid
and/or citric acid, and (C) a nonionic surface active agent and/or an
amphoteric surface active agent, in which the component (C) is mixed with
the components (A) and/or (B), or is provided separately, and at least
one substance selected from the particular metallic salts is contained in
at least one of the components (A), (B) and (C) in an amount of 5 to 25%
by weight.