The present invention relates to phytases having at least 76% identity to
a phytase derived from Hafnia alvei and comprises at least one
modification in the amino acid sequence thereof. These phytase variants
have modified, preferably improved, properties, such as, reduced protease
sensibility, preferably they exhibit improved properties in respect of
thermal performance, such as heat-stability (temperature stability,
thermostability), steam stability, pelleting stability and/or temperature
profile; and/or protease stability, in particular pepsin stability, pH
profile, specific activity, substrate specificity, performance in animal
feed (such as an improved release and/or degradation of phytate),
susceptibility to glycation, and/or glycosylation pattern. The invention
also relates to DNA encoding these phytases, methods of their production,
as well as the use thereof, e.g., in animal feed and animal feed
additives.