A phenol-free method of isolating DNA from biological material includes homogenizing a biological material with a homogenization buffer to form a homogenate. Proteins and non-DNA organic molecules are extracted from the homogenate by mixing a first extraction buffer and a second extraction buffer with the homogenate. The first extraction buffer includes chloroform and an alcohol and the second extraction buffer includes a non-ionic protein solubilizer and an alcohol. DNA is precipitated from the mixture of homogenate, first extraction buffer and second extraction buffer and the DNA is recovered by sedimentation.