The invention provides nucleic acids, and variants and fragments thereof,
obtained from strains of Bacillus thuringiensis encoding
.delta.-endotoxins having pesticidal activity against pests of the order
Coleoptera. The invention further provides mutagenized nucleic acids that
have been modified to encode endotoxins having improved pesticidal
activity and/or altered pest specificity. Particular embodiments of the
invention provide isolated nucleic acids encoding pesticidal proteins,
expression cassettes comprising such nucleic acids, and transformed
plants and seeds comprising a nucleic acid of the invention. These
compositions find use in methods for controlling pests, especially plant
pests.