This invention relates to a novel Biomolecule Transduction Motif (BTM), Mph-1 peptide (Mph-1-BTM), which has the potential to transduce many biological response modifiers effectively into the cytoplasm, intracellular organelles or nucleus of prokaryotic or eukaryotic cells in vivo and in vitro, and the related technological methods using Mph-1-BTM. This Mph-1-BTM can be used in the development of new recombinant protein vaccines or DNA/RNA vaccines, gene and protein therapy, production of pharmacologically or medicinally useful proteins, or pharmaco-medicinal drug therapy.