The present invention relates to a method of identifying calcium binding sites in gamma-crystallin useful in calcium-based homeostasis for the management of Cataract, said method comprising steps of isolating gamma-crystallin from eye lens, studying binding of calcium to the isolated protein by both direct and indirect methods, identifying Greek key Motif as calcium-binding sites of the protein gamma crystallin with the said protein binding 4-mol eq of calcium having dissociation constant of 90 micromole.