The present invention relates to pharmaceutical compositions comprising
virus-like particles (VLPs) of HPV, said VLPs adsorbed to an aluminum
adjuvant, and an ISCOM-type adjuvant comprising a saponin, cholesterol,
and a phospholipid. In preferred embodiments, the aluminum adjuvant
comprises amorphous aluminum hydroxyphosphate sulfate. Another aspect of
the invention provides multi-dose HPV vaccine formulations comprising HPV
VLPs and an antimicrobial preservative selected from the group consisting
of: m-cresol, phenol and benzyl alcohol. Also provided are methods of
using the disclosed pharmaceutical compositions and formulations to
induce an immune response against HPV in a human patient and to prevent
HPV infection.