Provided is an expression vector capable of constitutive expression of a
foreign gene in a bacterium belonging to the genus Rhodococcus. The
vector is a constitutive expression vector for a bacterium belonging to
the genus Rhodococcus comprising: DNA comprising a nucleotide sequence of
a mutated TipA gene promoter where a mutation is incorporated into a -10
region sequence of a TipA gene promoter, the mutated TipA gene promoter
being capable of thiostrepton-independent and constitutive expression of
a gene located downstream thereof; a promoter sequence for the
constitutive expression of a foreign gene; a ribosome-binding site
sequence located downstream of the promoter sequence; and a
multiple-cloning site sequence capable of incorporating a foreign gene
therein, located downstream of the ribosome-binding site sequence.