A dispensing assembly adapted for dispensing gloves, which includes a dispenser. The dispenser has a housing which forms an inner surface and an internal compartment configured to hold a stack of gloves, and at least one dispensing opening. A stack of gloves disposed in the dispenser on a glove positioner and the glove positioner biases the stack of gloves toward the dispensing opening and a portion of the housing such that the glove positioner controls the stack of gloves and space in the internal compartment of the dispenser to prevent gloves from moving out of the stack and into the space to permit easy and reliable withdrawal of each glove therefrom.