This invention relates to methods and systems for in silico or
bioinformatic modeling of cellular metabolism. The invention includes
methods and systems for modeling cellular metabolism of an organism,
comprising constructing a flux balance analysis model, and applying
constraints to the flux balance analysis model, the constraints selected
from the set consisting of: qualitative kinetic information constraints,
qualitative regulatory information constraints, and differential DNA
microarray experimental data constraints. In addition, the present
invention provides for computational procedures for solving metabolic
problems.