The present application provides a method of reducing and/or removing
diglyceride from an edible oil, comprising admixing an edible oil with an
acyl acceptor substrate and a diglyceride: glycerol acyltransferase,
wherein the diglyceride:glycerol acyltransferase is characterized as an
enzyme which in an edible oil is capable of transferring an acyl group
from a diglyceride to glycerol. The diglyceride:glycerol acyltransferase
can comprise the amino acid sequence motif GDSX. The present invention
also relates to the use of a diglyceride:glycerol acyltransferase in the
manufacture of an edible oil, for reducing and/or removing diglyceride
from said edible oil and to the use of said enzyme in the manufacture of
a foodstuff comprising an edible oil for improving the crystallization
properties of said foodstuff.