The invention relates to a process of recovering galactose from a solution derived from plant-based biomass. In the process of the invention, the starting solution is subjected to one or more chromatographic fractionation steps, which may carried out with a strongly basic anion exchange resin and optionally with a strongly acid cation exchange resin, in any desired sequence, followed by recovering at least one fraction enriched in galactose. The galactose fraction thus obtained is further purified by crystallization to obtain crystalline galactose. The invention also relates to non-animal derived crystalline D-galactose.