The disclosed is thiol-modified macromolecule derivatives having the general formula of (I) or (II), as well as the disulfide bond cross-linked materials and the thiol-reactive crosslinker cross-linked materials, wherein R.sub.1 and R.sub.2 are alkylene groups, substituted alkylene groups, aromatic groups or polyether groups and the like, respectively. R.sub.1 and R.sub.2 may have same or different chemical structure, and P is a residue of a macromolecule with side carboxyl group. The thiol-modified macromolecule derivative has a molecular weight of from 1000 to 5,000,000. The thiol-modified macromolecule derivative having the general formula of (I) or (II) of the present invention has a side chain with flexible chemical structure and adjustable properties, and has a number of advantages, such as mild reaction condition, high production yield, high degree of modifying, and the controllable modifying degree and so on. The cross-linked material made from the thiol-modified macromolecule derivative of the present invention can be used to inhibit cell attachment and be used as the matrix for cell attachment growth. ##STR00001##