A dispenser for dispensing gloves is provided which includes a housing having an exit port, the housing also formed to include a compartment which is configured to hold a plurality of gloves therein. The dispenser includes a glove pusher movably coupled to the housing. At least a portion of the glove pusher is configured to move within the compartment of the housing and push at least a portion of a glove disposed in the compartment through the exit port. A cartridge configured to be disposed into a compartment of a dispenser is disclosed. The cartridge is formed to permit a portion of a dispenser to move into the internal compartment of the cartridge to move at least one of the plurality of gloves at least partially through the at least one opening in the cartridge. A dispensing assembly also may include a stack of gloves or a cartridge containing a plurality of gloves. A method of using a glove dispenser is also provided.