The present invention provides a method and system for progressive multi-resolution three-dimensional image reconstruction using region of interest information. The method and system significantly reduces the amount of time required to display high quality three-dimensional fluoroscopic images by utilizing progressive multi-resolution image reconstruction techniques. In addition, by employing manual or automated selection of one or more regions of interest in which to focus the progressive image reconstruction, high quality 3D images of region(s) of interest may be obtained soon after image data acquisition.