The present invention provides methods of detection, including early
detection, for cancer or other diseases and normal physiologic processes
mediated by global epigenetic changes, by using one or more of the
following biomarkers: a global DNA methylation index, a global histone H4
acetylation index, and a global histone H4 trimethylation index. These
methods are useful for, among other things, assessing the effectiveness
of treatment, monitoring relapse, and clinical staging of cancer and
other chronic as well as acute diseases. These methods are also useful
for among other things monitoring the effectiveness of strategies and
therapies used to modify lifestyle and contextual effects to prevent
disease, foster wellness and enable health promotion.