Method for detecting and identifying unknown bioagents, including
bacteria, viruses and the like, by a combination of nucleic acid
amplification and molecular weight determination using primers which
hybridize to conserved sequence regions of nucleic acids derived from a
bioagent and which bracket variable sequence regions that uniquely
identify the bioagent. The result is a "base composition signature" (BCS)
which is then matched against a database of base composition signatures,
by which the bioagent is identified.