The utility model of a dental floss holder concerns a device to handle dental floss to clean the spaces between the teeth consisting of two interconnected but easily separable holder portions: Portion 1--U shape supporter is provided with a U supporter (1) with two folding legs, connecting part (2) with longitudinal groove (6) and external thread (7), a hole (3) in the rear leg, a groove (4) that goes externally from the end of the front leg to the fixed end of U supporter (1) to hold the dental floss in position during cleaning operation. Portion 2--Hollow grip provided with a fixed side with a longitudinal inner blade (8) and the rotating side with a rotary end (9) and an internal thread (10). These two portions constitute a mechanism that limits the maximum floss tension adjusted by the rotary end (9). The device can operate with a piece of floss or from a floss supplier (12) connected to the rotary end (9).