The invention relates to a method for producing a Cucumis sativus plant,
providing cucumber fruits exhibiting the phenotypic trait "extended shelf
life," comprising: (a) providing a first Cucumis sativus plant containing
at least one quantitative trait locus (QTL) associated with said
phenotypic trait; (b) crossing the plant provided in step (a) with a
second Cucumis sativus plant; (c) analyzing the progeny of the cross of
step (b) for the presence or absence of said QTL; and (d) identifying and
selecting plants containing said QTL; wherein the at least one QTL is
selected from QTL1 or QTL2, and wherein QTL1 is identified by a first
molecular marker of about 66 base pairs consisting from 5' to 3' of a
first primer having the nucleotide sequence SEQ ID No:1, a cucumber
genomic fragment, and a second primer having the nucleotide sequence SEQ
ID No:2, and a second molecular marker of about 230 base pairs consisting
from 5' to 3' of a first primer having the nucleotide sequence SEQ ID
No:3, a cucumber genomic fragment, and a second primer having the
nucleotide sequence SEQ ID No:4, or any part of the DNA sequence between
said first and second marker; and QTL2 is identified by a first molecular
marker of about 137 base pairs consisting from 5' to 3' of a first primer
having the nucleotide sequence SEQ ID No:5, a cucumber genomic fragment,
and a second primer having the nucleotide sequence SEQ ID No:6, and a
second molecular marker of about 230 base pairs consisting from 5' to 3'
of a first primer having the nucleotide sequence SEQ ID No:7, a cucumber
genomic fragment, and a second primer having the nucleotide sequence SEQ
ID No:8 or any part of the DNA sequence between said first and second
marker.