Methods for biodegrading nitroaromatic compounds present as contaminants in
soil or water using microorganisms are disclosed. Water is treatable
directly; dry soil is first converted into a fluid medium by addition of
water. The preferred method comprises two stages, each employing
microorganisms: a fermentative stage, followed by an anaerobic stage. The
fermentative stage is rapid, wherein an inoculum of aerobic and/or
facultative microorganisms ferments a carbohydrate added to the fluid
medium, exhausting the oxygen in the fluid medium and thereby inhibiting
oxidative polymerization of amino by-products of the nitroaromatics. In
the subsequent anaerobic stage, an inoculum of a mixed population of
anaerobic microorganisms completes the mineralization of the contaminant
nitroaromatics, using the remaining carbohydrate as a carbon and energy
source. Preferably, the carbohydrate is a starch and the aerobic and/or
facultative microorganisms are amylolytic, which cleave the starch at a
moderate rate throughout both stages, ensuring a sustained supply of
metabolizable carbohydrate. The microorganisms are preferably selected to
be resistant to the types and concentrations of nitroaromatics present as
contaminants.