Two stable cytolytic T lymphocyte cell lines and a clone are established from two primary colorectal carcinoma patients. A method for generating stable anti-colorectal carcinoma CTL clones and cell lines includes the step of stimulating the lymphocytes of a patient with minimal or no clinical evidence of colorectal carcinoma in culture with irradiated autologous primary colorectal carcinoma tumor cells, interleukin-2, and either autologous lymphocytes or autologous EBV-B cells.