The invention relates to a method to increase protein expression in baculo vector virus expression systems. The invention provides a method to produce a recombinant protein in insect-cell culture which comprises selecting a recombinant baculovirus expressing said protein, growing insect cells in growth medium in a culture vessel and infecting the cells with an inoculum of at least one baculovirus at a cell density of 1.times.10.sup.5 to 5.times.10.sup.6 cells/ml with an m.o.i of <0.01. The invention furthermore provides a method to produce recombinant pestivirus E2 or E.sup.m9 protein or fragments thereof in insect cell culture characterized by a final concentration of said protein (fragments) in the growth medium at harvest of at least 100 .mu.g/ml. The invention furthermore provides a method to produce recombinant follicle stimulating hormone, .alpha.-units an/or .beta.-units and complexes and fragments thereof, at a concentration in the growth medium at harvest of at least 15 .mu.l.

Die Erfindung bezieht auf einer Methode auf Zunahmeproteinausdruck in den baculo vektorvirus-Ausdruck Systemen. Die Erfindung liefert eine Methode, um ein recombinant Protein in der Insekt-Zelle Kultur zu produzieren, dessen das Vorwählen eines recombinant baculovirus enthält, das besagtes Protein ausdrückt, Insektzellen im Wachstummittel in einem Kulturbehälter wächst und die Zellen mit einem Impfstoff von mindestens einem baculovirus an einer Zelle Dichte von 1.times.10.sup.5 zu 5.times.10.sup.6 cells/ml mit m.o.i ansteckt