Measuring esterase activity using fluorescent substrates as a way of evaluating cervical cancer

   
   

The present invention provides methods of determining whether test cells in a sample are cancerous or not and determining if lymphocytes are activated or not. The method measures the test cell's DNA ploidy and the cellular activity of an enzyme such as an esterase, which has altered expression in cancer cells. Esterase activity can be measured using fluorescent compounds such as fluorescein diacetate.

La actual invención proporciona métodos de determinar si las células de la prueba en una muestra son cancerosas o no y determinándose si se activan los linfocitos o no. El método mide la DNA de la célula de la prueba ploidy y la actividad celular de una enzima tal como un esterase, que ha alterado la expresión en células de cáncer. La actividad del esterase se puede medir usando compuestos fluorescentes tales como diacetate del fluorescein.

 
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