This invention is directed toward a peptide corresponding to an
immunologically important viral epitope. Specifically, the peptide
corresponds to an immunodominant epitope identified in the envelope region
of the human immunodeficiency virus type 1 (HIV-1). This peptide has the
following amino acid sequence: NH.sub.2
-Asn-Asn-Thr-Arg-Arg-Gly-Ile-His-Met-Gly-Trp-Gly-Arg-Thr-Phe-Tyr-Ala-Thr-G
ly-Glu-Ile-Ile-Gly-CO.sub.2 H (SEQ ID NO:17). The invention also relates to
the use of this peptide, particularly when biotinylated in the form of
complexes of streptavidin-biotinylated peptides or of avidin-biotinylated
peptides, for the in vitro determination of HIV-1-specific antibodies.
Esta invenção é dirigida para um peptide que corresponde a um epitope viral immunologically importante. Especificamente, o peptide corresponde a um epitope immunodominant identificado na região do envelope do tipo humano 1 do vírus do immunodeficiency (HIV-1). Este peptide tem a seguinte seqüência do amino-ácido: NH.sub.2 - Asn-Asn-Thr-Arg-Arg-Gly-Ile-Seu-Encontr com-Gly-Trp-Gly-Arg-Thr-Phe-Tyr-Ala-Asn-Asn-Thr-Arg-Arg-Gly-Ile-His-Met-Gly-Trp-Gly-Arg-Thr-Phe-Tyr-Ala-Thr-G-G ly-Glu-Ile-Ile-Gly-CO.sub.2 H (Id SEQ NO:17). A invenção relaciona-se também ao uso deste peptide, particularmente quando biotinylated no formulário dos complexos de streptavidin-biotinylated peptides ou de peptides do avidin-biotinylated-biotinylated, para na determinação de vitro de antibodies de HIV-1-specific.
